A Cold-Inducible RNA Binding Protein Gene from Acanthopagrus schlegelii: Molecular Characterization, Expression, and Association with Apoptosis to Low-Temperature Stress

In this study, the complete cDNA sequence (1552 bp) of cold-inducible RNA binding protein gene (cirbp gene) was successfully cloned from liver in Acanthopagrus schlegelii (initial weight: 15.0 ± 2.3 g). Results showed that Ascirbp A. schlegelii) has 24 phosphorylation sites, no signal peptide, and transmembrane helix structure. AsCIRBP, with a molecular weight 18.84 ku an isoelectric point 9.04 stable encodes 182 amino acids. Subcellular localization analysis it located nucleus. Sequence alignment results AsCIRBP acid sequences various fishes including black porgy were highly conserved, especially recognition motif (RRM). Those real-time quantitative PCR (qRT-PCR) demonstrated specifically expressed tissue its expression significantly increased under cold stress or acclimation. The interference experiment Ascirbp-dsRNA could suppress through intraperitoneal injection. After silencing gene, RNAi groups more severely damaged structure prone to apoptosis than control groups. study on linkage between mitochondrial pathways changes had significant effect key genes apoptosis. most striking result expressions bcl-2 apaf-1 decreased significantly, which can block normal apoptotic process. disruption process, p53, bax, cyto-c, caspase-9, caspase-3, diablo, caspase-1 affected. These suggest inhibit protect at low temperatures.